Tailored preclinical solutions
CRO Services
Delivering customized preclinical services to accelerate drug development with precision, reliability, and expertise in metabolic research.
CRO SERVICES
In vivo services
Body Measurements
Body weight food/water intake, organs collection, weight and fixation (fat pads, liver, etc.), blood collection and tissue dissection.
Energy Expenditure
Indirect calorimetry measures energy expenditure by assessing oxygen consumption and carbon dioxide production, providing insight into metabolic rates.
Body Composition
Body composition via nuclear magnetic resonance analyzes fat and muscle distribution, helping monitor changes in health and fitness.
Behavioral tests
Metabotech offers a wide variety of tests to asses parameters as: memory, stress, anxiety, learning, spontaneous activity, balance, grip strength, endurance, motor coordination, and physical resistance.
Oncology
Xenograft experiments in immunodeficient mice using tumor cell lines with the aim of generating tumors for further validation studies of tumor initiation capacity, tumor growth assessment, pharmacological efficacy studies and survival rate evaluation.
CRO SERVICES
In vivo drug efficacy experiments
In vivo metabolic studies are a mandatory step for the development of drugs targeting metabolic disorders. Using our different mice models, we deliver golden – standard and innovative experiments to demonstrate your drugs’ efficacy. Benefit from our validated radio-tracer based experiments and technical platform to dissect the mechanism by which your drug treats metabolic disorders
Energy homeostasis
- Assessment of body composition (Nuclear magnetic resonance”.).
- Assessment of energy expenditure (Sable System): 36 climatic chambers with control of ambient temperature (from 4 to 30ºC) and control of light/dark cycles.
- Bomb calorimetry.
- Infrarred camera.
Glucose homeostasis
- Euglycemic hyperinsulinemic clamp
- Hyperglycemic clamp
- Hypoglycemic clamp
- Whole body glucose turnover
- In vivo tissue-specific glucose uptake<
- Hepatic glucose production
- Glucose tolerance test – GTT
- Insulin tolerance test – ITT
- Pyruvate tolerance test- PTT
- Glycerol tolerance test-GlyTT
- Glucose-stimulated insulin secretion- GSIS
Lipid metabolism
In vivo metabolic studies are a mandatory step for the development of drugs targeting metabolic disorders. Using our different mice models, we deliver golden – standard and innovative experiments to demonstrate your drugs’ efficacy. Benefit from our validated radio-tracer based experiments and technical platform to dissect the mechanism by which your drug treats metabolic disorders.
Oncology
- Tumor Initiation Capacity
- Tumor Growth Evaluation
- Pharmacological Efficacy Studies
- Survival Rate Assessment
CRO SERVICES
Surgery services
- Catheter implantation into the jugular vein
- Catheter implantation into the carotid artery
- Injection of substances into the tail vein
- Orchiectomy / Ovariectomy
- Implantation of osmotic pumps
- Stereotaxic surgery
- Cava vein insulin administration
- Splenic puncture
CRO SERVICES
Biochemical and histological analysis
These are complementary methodologies alongside in vivo/ex vivo studies to comprehensively characterize the potential of your drug candidates and their tissue-level impact within organisms.
Albor Biotech offers an extensive array of biochemical assays and histology analyses to evaluate the efficacy of your drugs/genes.
ELISAS and enzimatic assays
- Insulin
- Glucagon
- Leptin
- Others
Western Blot analysis
For detection and quantification of protein levels in samples/tissues.
Real time PCR (qPCR)
For detection and quantification of gene expression in any tissue.
RNA scope
For detection and quantification of gene expression in any tissue.
Histology service
Optic or confocal microscopy of several stainings (H&E, ORO, Sirius Red, etc.), immunohistochemestry and immunofluorescence.
CRO SERVICES
In vitro / ex vivo assays
These kind of assays are a valuable tool in scientific research that allow researchers to conduct controlled experiments to understand a wide range of biological processes and develop new medical treatments and therapies. We offer a realm of possibilities, including Seahorse Technology, to address your in vitro pharmacology needs, enhancing the efficacy of the drug or gene testing.
Metabolic Parameters
- MItochondrial respiration (Seahorse technology)
- Lipid overload
- Cell sorting
- Primary hepatocyte culture
- Oxidative stress assays
- Proliferation assays
- Survival assays
- Enzymatic assays
- Treatments/transfections
- Protein/gene analysis
Oncology
- Cell proliferation/viability assays (MTT assay)
- 3D Cell Culture/Spheroid Experiments (tumor growth)
- Apoptosis assays: apoptosis induction assessment by flow cytometry or
- markers
- Cell cycle assays: cell cycle evaluation by flow cytometry or markers
- Colony formation assays
- Cell migration, invasion and extravasation assays
- Inmunofluorescence and inmunohistochemestry techniques for tumor
- marker detection
- Protein/gene expression analysis
CRO SERVICES
Diet- and chemical-induced obese models
Conducting in vivo metabolic studies is an essential prerequisite in drug development for addressing metabolic disorders. Using our different chemical- or diet-induced obese/fibrotic models, we offer both gold-standard and innovative experiments to effectively showcase the efficacy of your drugs.
